Morphological and Apoptotic Comparison of Primordial and Primary Follicles in Cryopreserved Human Ovarian Tissue     

LH Wang  SF Mullen  Y Li  JQ Zhong  JK Crister  ZJ Chen 《Reproduction in domestic animals》2009,44(6):879-883

There are few reports which were designed to compare the survival rate of human primary follicles with primordial follicles after cryopreservation. This study was designed to evaluate whether such a difference occurs. Human ovarian biopsies were cryopreserved using dimethylsulphoxide/sucrose as the cryoprotectants. Fresh and cryopreserved ovarian samples were evaluated for viability differences between the two types of follicles using the endpoints of histology, ultrastructure and DNA fragmentation. In comparison with fresh ovarian tissue (83.9% ± 10.0%), the percentage of morphologically normal primordial follicles was not significantly different in cryopreserved tissue (73.9% ± 17.2%). However, a lower percentage of primary follicles with normal morphology was seen in the cryopreserved group (43.3% ± 25.7% vs 74.8% ± 19.4% for the fresh group). Transmission electron microscopy revealed that the cryopreservation did not appear to affect the structural integrity of primordial follicles; however, varying ultrastructural damage to the cytoplasm was observed in the majority of the cryopreserved primary follicles. Using a DNA fragmentation assay, the percentage of apoptotic primordial and primary follicles in the unfrozen (26.3% and 20%) and frozen (23.3% and 25%) ovarian tissue was similar. A higher proportion of primary follicles, compared to primordial follicles, suffer histological damage after slow freezing.  相似文献   

The effect of calcium and phosphorus supplementation, inactivity, and subsequent aerobic training on the mineral balance in young, mature, and aged horses     

M.A. Buchholz-Bryant MS  L.A. Baker PhD  J.L. Pipkin PhD  B.J. Mansell MS  J.C. Haliburton DVM  PhD  R.C. Bachman MS 《Journal of Equine Veterinary Science》2001,21(2)

A randomized complete block design experiment was conducted to determine the effect of Ca and P supplementation, inactivity, and subsequent aerobic exercise on Ca and P serum concentrations, and balance and digestibilities in young, mature, and aged horses. Twelve stock-type geldings were blocked into three age groups: 2 and 3, 7 to 11, and 15 to 21 years of age. Two groups of six horses were randomly assigned to either control (C) or mineral supplemented (T) diet. Horses consuming C received about 133% of National Research Council (NRC) requirements for Ca and P, while horses consuming T received about 275% of NRC requirements for Ca and P. The trial consisted of eight 21-d periods. During periods I, II, and III, all horses were inactive, and during periods IV, V, VI, VII, and VIII, all horses performed an ascending aerobic exercise regimen. Serum samples were taken at the beginning of period I and at 21-d intervals thereafter for determination of serum Ca and P. Total fecal and urine collections were taken for 72 h at the beginning of period I and at 21-d intervals thereafter for determination of Ca and P balance. Overall mean apparent Ca balance was affected by treatment (P < .009); time (P < .001); and the interaction of time and treatment (P < .001). Overall mean apparent Ca balance was higher (P < .009) for T as compared to C (23.63 vs. 3.77 mg/kg BW). Mean apparent Ca digestibility was affected by time (P < .001) and the interaction of time and age (P < .001). Mean apparent P balance was affected by time (P < .001) and the interaction of time and treatment (P < .032). Increases in Ca balance may indicate more available mineral for bone metabolism, particularly when remodeling is triggered with the onset of exercise following a sedentary period, suggesting a possible benefit to feeding additional Ca above current NRC recommendations.  相似文献   

Haemophilia A in German shepherd dogs   总被引：1，自引：0，他引：1  

B W Parry  M A Howard  P D Mansell  S A Holloway 《Australian veterinary journal》1988,65(9):276-279

Haemophilia A was diagnosed in 14 male German shepherd dogs. Factor VIII: coagulant (FVIII:C) activities ranged from 1.13% of a normal canine plasma pool. von Willebrand's factor antigen values were normal or increased in all 9 of these dogs which were tested. Twelve of these dogs had a common maternal grandsire. Five dogs had exhibited no tendency to bleed (when tested between 2 to 23 months of age). They were tested because of pedigree links with clinically affected animals. Common clinical signs in the latter dogs included: bleeding from the mouth, subcutaneous and intramuscular haematomas and lameness. Since these dogs usually had a mild to moderate deficiency of FVIII:C, they may survive to adulthood without exhibiting clinical signs severe enough to necessitate veterinary attention.  相似文献   

The use of a hand-held conductivity meter for the diagnosis of subclinical mastitis in dairy cows during late lactation     

Mansell PD  Seguya A 《New Zealand veterinary journal》2003,51(1):21-25

AIM: To evaluate the accuracy of a hand-held electrical conductivity meter for the detection of subclinical mastitis in an Australian dairy herd in late lactation. METHODS: A hand-held conductivity meter was evaluated during late lactation in a herd of dairy cows that had a high prevalence of Streptococcus agalactiae and Staphylococcus aureus infection. The ability of the conductivity meter to accurately discriminate between uninfected quarters and those infected with major pathogens was assessed, using bacteriology as the definitive test for infection status. Milk samples for bacteriology, and electrical conductivity measurements, were collected from 233 quarters from 59 cows. The ability of the device to identify infected and uninfected cows was also assessed. Sensitivity and specificity were calculated for a range of threshold values, using absolute values, the range of quarter values within a cow and the ratio of quarter values within a cow. RESULTS: Electrical conductivity was higher in infected quarters than uninfected quarters, but the degree of overlap between the populations was substantial, even when quarters were assessed as case-control pairs from the same cow. Use of the device according to the manufacturer's recommendations for diagnostic criteria of mastitic quarters had a sensitivity of 51% and a specificity of 71%. The ability of the device to correctly diagnose the infection status of cows was dependent on both the analytical method and the threshold value used. Applying the manufacturer's recommended criteria to the diagnosis of the status of cows, the sensitivity of the test was 91% and the specificity 17%. CONCLUSION: The variability between individual quarters and between cows in this study was such that the use of this device to measure the conductivity of milk was not a reliable method by which to diagnose subclinical mastitis in cows in late lactation. CLINICAL RELEVANCE: Interpretation of results from handheld conductivity meters when used for the diagnosis of subclinical mastitis in dairy cows needs to be made with care. In some circumstances the results obtained do not accurately reflect the bacteriological status of either individual quarters, or cows.  相似文献   

Changes in Factor VIII:Coagulant Activity and von Willebrand Factor Antigen Concentration After Subcutaneous Injection of Desmopressin in Dogs with Mild Hemophilia A     

Peter D. Mansell BVSc  Bruce W. Parry BVSc  PhD 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1991,5(3):191-194

Four German Shepherd dogs with mild hemophilia A were given Desmopressin (Minirin intranasal solution, Ferring, Malmo, Sweden) subcutaneously at a range of doses in a controlled blind study. No substantial change in plasma FVIII activity was observed 0.5, 1, 2, 4, or 6 hours after administration of Desmopressin. Plasma von Willebrand factor antigen concentrations increased rapidly after doses of 0.4, 1.0, 2.0, and 5.0 micrograms/kg to mean values of 140%, 127%, 120%, and 140% of baseline, respectively. The duration of this increase was dependent on the dose of Desmopressin injected.  相似文献   

Hormonal Response of Female Goats to Active Immunization against a Recombinant Human Inhibin α-subunit, and establishment of an Enzyme-linked Immunosorbent Assay for Caprine Follicle-stimulating Hormone     

M Hennies  JK Voglmayr  E Dietrich  M Stollmann  R Moeller  & W Holtz 《Reproduction in domestic animals》2001,36(2):65-71

The effect of selective immunosuppression of endogenous inhibin in goats on FSH, LH, progesterone and estradiol-17β profiles was studied during the breeding and nonbreeding seasons. Eighteen adult female Boer goats were immunized against the recombinant human inhibin α-subunit (hINH-α). With the exception of estradiol, which was determined by radio-immunoassay (RIA), all plasma hormone concentrations were determined by ELISA. The ELISA for FSH presented in this paper was established in the authors' laboratory, based on an existing RIA. Mean basal concentrations of FSH were not affected by immunosuppression of endogenous inhibin, nor was there a difference in the amplitude of the pre-ovulatory FSH surge. Immunization against inhibin appears to eliminate the slight secondary rise of FSH occurring 12–20 h after the major surge associated with ovulation. The LH profiles of the immunized goats were characterized by lower basal concentrations both before and after the pre-ovulatory LH surge which itself was reduced by 50% in immunized does. By contrast, concentrations of circulating estradiol were significantly elevated after inhibin-immunization. Progesterone profiles were not affected. Extending immunization into the anoestrous season by a booster injection of hINH-α, implicating oestrus induction with a progestagen and eCG, produced no discernible differences in FSH and LH profiles in comparison with nonimmunized control goats. The findings suggest that in goats, paracrine factors may play a more significant role in controlling follicular activity than a feedback mechanism acting via the pituitary.  相似文献   

Survival data for canine oral extramedullary plasmacytomas: a retrospective analysis (1996-2006)     

Wright ZM  Rogers KS  Mansell J 《Journal of the American Animal Hospital Association》2008,44(2):75-81

In a 10-year period, extramedullary plasmacytomas (EMP) represented 5.2% of all oral tumors found in the dog (16/302). These 16 oral EMP comprised 28.5% of all EMP within the same time period. Eleven dogs died with a median survival time of 474 days. Five dogs remain alive at the time of this writing. Dogs without complete surgical removal of the EMP and no adjuvant therapy had a median survival time of 138 days. Oral EMP have a clinical behavior consistent with EMP arising from other tissues. They have no obvious correlation with multiple myeloma, and complete surgical resection may be curative.  相似文献   

Phosphorus deficiency in a dairy herd     

Brooks HV  Cook TG  Mansell GP  Walker GA 《New Zealand veterinary journal》1984,32(10):174-176

Phosphorus deficiency was diagnosed in a 90 cow seasonal supply dairy herd which showed low milk production, ill-thrift, infertility and osteophagia. Serum inorganic phosphorus and pasture phosphorus levels were low, pasture Ca:P ratios high and soil phosphorus levels very low; the soil phosphate retention value was 96%. The deficiency, which had probably existed for several years, was considered to be due to inadequate annual phosphate fertiliser applications on a high phosphate retaining soil. Phosphorus deficiency may have been exacerbated by the application of lime without phosphate several months before the onset of severe clinical disease. The finding of low sodium levels in pasture samples suggested that sodium deficiency may have co-existed and contributed to the clinical picture. Treatment, comprising bone flour dusting of pasture, water trough supplementation and phosphorus-containing injections, appeared to induce ovarian activity in anoestrous cows and suppress osteophagia, though controlled treatment trials were not performed. No improvement was noted in milk production or cow condition. Increased annual super-phosphate and reduced potassium applications were recommended, together with the monitoring of pasture and soil macro elements and serum phosphorus levels. A decision on whether to supplement with sodium would be based on the results of pasture monitoring, since a direct animal test is not currently available. It is suggested that phosphorus deficiency may not be uncommon in dairy herds in some North Island districts; based on the present case, the decline in New Zealand superphosphate quality in the 1970's and recently published data which has shown a high proportion of pasture phosphorus deficient sites in some North Island areas.  相似文献   

A survey of northern Victorian dairy farmers to investigate dairy calf management: calf‐rearing practices          下载免费PDF全文

AJ Phipps  DS Beggs  AJ Murray  PD Mansell  MF Pyman 《Australian veterinary journal》2018,96(4):107-110

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First detection of bovine noroviruses and detection of bovine coronavirus in Australian dairy cattle          下载免费PDF全文

SJ Symes  JL Allen  PD Mansell  KL Woodward  KE Bailey  JR Gilkerson  GF Browning 《Australian veterinary journal》2018,96(6):203-208

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